Detection of Ang II, Ang-(1-7) Concentrations and NAGase Activity
The Ang II and Ang-(1-7) concentrations in the cell culture supernatant was calculated via ELISA (Hengyuan Biotechnology Co, Shanghai, China). ELISA test was conducted according to the manufacturer’s instructions. The concentration of Ang II and Ang-(1-7) was expressed as ng/L. The activity of N-acetyl-b-Dglucosaminidase (NAGase) in the cell culture supernatant was determined according to the instructions of the kit, which was purchased from the Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The level of the activity of NAGase was
