【文獻(xiàn)標(biāo)題】Efects of electroacupuncture on the kisspeptin-gonadotropin-releasing hormone (GnRH) /luteinizing hormone (LH) neural circuit abnormalities and androgen receptor expression of kisspeptin/neurokinin B/dynorphin neurons in PCOS rats
【作者】Ge Xu, Xuedan Zhao, Zhihao Li,et.al
【作者單位】上海中醫(yī)藥大學(xué)(Shanghai University of Traditional Chinese Medicine)
Te serum concentrations of T, E2, and LH and hypothalamic ARC levels of kisspeptin, GnRH, Dyn, and NKB were quantifed using rat ELISA kits (Shanghai Hengyuan Biological Co., Ltd., Shanghai, China). All experiments were performed according to the manufacturer’s instructions. Te kit detection range was 5–160nmol/L for T, 3–80ng/L for E2, 1.5–40ng/L for LH, 10–350pg/mL for kisspeptin, 1–48ng/L for GnRH, 0.2–9μg/L for NKB, and 12–500pg/mL for Dyn.