【文獻標題】Ginsenoside Rd promotes omentin secretion in adipose through TBK1-AMPK to improve mitochondrial biogenesis via WNT5A/Ca2+pathways in heart failure
【作者】Shiyao Wan , ZeKun Cui , Lingling Wu ,et.al
【作者單位】中國藥科大學(xué)( China Pharmaceutical University)
After the observation, blood samples were collected from mice and serum were separated. According to the instructions, the contents of Nterminal pro-B type natriuretic peptide (NT-proBNP, HB533-Mu, Hengyuan, Shanghai, China), malondialdehyde (MDA, A003-1-2, Jiancheng, Nanjing, China), lactate dehydrogenase (LDH, A020-2-2, Hengyuan, Shanghai, China) and omentin (HB310-Mu, Hengyuan, Shanghai, China) were determined by ELISA kit. Adipokines released by 3T3-L1 adipocytes were detected by TNF-α (HB049-Mu, Hengyuan, Shanghai, China) Elisa kits.